Back to Search
Start Over
Enhanced viability of a nervous necrosis virus-infected stable cell line over-expressing a fusion product of the zfBcl-xL and green fluorescent protein genes.
- Source :
-
Journal of Fish Diseases . Jun2006, Vol. 29 Issue 6, p347-354. 8p. 3 Diagrams, 2 Graphs. - Publication Year :
- 2006
-
Abstract
- Nervous necrosis virus (NNV) infection induces host cell apoptosis by an ill-understood process. We utilized a fusion between enhanced green fluorescent protein (EGFP) and the zfBcl-x L gene in GL-av cells to select for zfBcl-xL stable cell lines and to assess the effectiveness of the anti-apoptotic protein Bcl-xL in circumventing NNV-induced cell death. Stable EGFP and EGFP-Bcl-xL-expressing clones were obtained at high purity within 2.5–3 months. In the latter, the EGFP-Bcl-xL fusion protein (approximately 58.2 kDa, as ascertained by Western blot) was predominantly targeted to mitochondria. We assayed for apoptosis in red-spotted grouper NNV Tainan no. 1 (RGNNV TN1)-infected cells with terminal deoxynucleotidyl transferase (TdT)-mediated end labelling (TUNEL) of DNA at different virus doses. NNV infection of NNV Bcl-xL GL-av cell line revealed a protective effect, with a decrease in TUNEL-positive cells of 7%, 8% and 31.8% at 24, 48 and 72 h, respectively. In addition, RGNNV infection of the Bcl-xL GL-av cell line revealed a protective effect, with an enhanced viability of 3%, 40% and 73% at 24, 48, and 72 h, respectively. We conclude that NNV-induced apoptotic cell death can be lessened in transgenic grouper fish cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *APOPTOSIS
*GREEN fluorescent protein
*NECROSIS
*VIRUS diseases
Subjects
Details
- Language :
- English
- ISSN :
- 01407775
- Volume :
- 29
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Fish Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 21063944
- Full Text :
- https://doi.org/10.1111/j.1365-2761.2006.00725.x