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Interaction between β-lactoglobulin and lactase and its effect on enzymatic activity
- Source :
-
International Dairy Journal . Oct2006, Vol. 16 Issue 10, p1169-1173. 5p. - Publication Year :
- 2006
-
Abstract
- Abstract: The activity of Kluyveromyces lactis β-galactosidase increased when it was measured in the presence of either β-lactoglobulin or bovine serum albumin, whereas α-lactalbumin showed no effect on enzyme activity. Heating β-lactoglobulin further increased β-galactosidase activity. Affinity chromatography assays demonstrated that lactase bound specifically to β-lactoglobulin, resulting in enzyme activation. Heating β-lactoglobulin in the presence of lactose resulted in a reaction between the protein and the sugar (lactolation), which diminished the binding capacity of the protein to the enzyme and therefore its activating effect. It was concluded that β-lactoglobulin raises lactase activity through two different mechanisms, one of which depends on the release of sulfhydryl groups by heat treatment from the denatured protein, and the other a result of the ability of the native protein to bind the enzyme. [Copyright &y& Elsevier]
- Subjects :
- *KLUYVEROMYCES
*SACCHAROMYCETACEAE
*CHROMATOGRAPHIC analysis
*SERUM albumin
Subjects
Details
- Language :
- English
- ISSN :
- 09586946
- Volume :
- 16
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- International Dairy Journal
- Publication Type :
- Academic Journal
- Accession number :
- 21188108
- Full Text :
- https://doi.org/10.1016/j.idairyj.2005.10.003