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Divalent metal dependence of site-specific DNA binding by ecoRV endonuclease.

Authors :
Martin, Amy M.
Horton, Nancy C.
Source :
Biochemistry. 06/29/1999, Vol. 38 Issue 26, p8430. 10p. 2 Black and White Photographs, 2 Charts, 4 Graphs.
Publication Year :
1999

Abstract

Suggests that the measurements of DNA binding affinities for EcoRV endonuclease should be performed in the presence of divalent metal ions. Enhancement of expression of sequence selectivity in the presence of Ca2+; Equilibrium dissociation constants of wild-type and base-analogue sites.

Subjects

Subjects :
*ENDONUCLEASES
*DNA

Details

Language :
English
ISSN :
00062960
Volume :
38
Issue :
26
Database :
Academic Search Index
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
2170931
Full Text :
https://doi.org/10.1021/bi9905359