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Inducible and reversible suppression of Npm1 gene expression using stably integrated small interfering RNA vector in mouse embryonic stem cells
- Source :
-
Biochemical & Biophysical Research Communications . Sep2006, Vol. 347 Issue 4, p1129-1137. 9p. - Publication Year :
- 2006
-
Abstract
- Abstract: The tetracycline (Tc)-inducible small interference RNA (siRNA) is a powerful tool for studying gene function in mammalian cells. However, the system is infrequently utilized in embryonic stem (ES) cells. Here, we present the first application of the Tc-inducible, stably integrated plasmid-based siRNA system in mouse ES cells to down-regulate expression of Npm1, an essential gene for embryonic development. The physiological role of Npm1 in ES cells has not been defined. Our data show that the knock-down of Npm1 expression by this siRNA system was not only highly efficient, but also Tc- dose- and induction time-dependent. Particularly, the down-regulation of Npm1 expression was reversible. Importantly, suppression of Npm1 expression in ES cells resulted in reduced cell proliferation. Taken together, this system allows for studying gene function in a highly controlled manner, otherwise difficult to achieve in ES cells. Moreover, our results demonstrate that Npm1 is essential for ES cell proliferation. [Copyright &y& Elsevier]
- Subjects :
- *RNA
*CELL proliferation
*CELL division
*MOBILE genetic elements
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 347
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 21775550
- Full Text :
- https://doi.org/10.1016/j.bbrc.2006.07.020