Back to Search
Start Over
Silencing of HIV-1 Gene Expression by Sirnas in Transduced Cells.
- Source :
-
Nucleosides, Nucleotides & Nucleic Acids . Jul2006, Vol. 25 Issue 7, p795-799. 4p. - Publication Year :
- 2006
-
Abstract
- The RNA interference (RNAi) phenomenon is a recently observed process in which the introduction of a double-stranded RNA (dsRNA) into cells causes the specific degradation of an mRNA containing the same sequence. To study dsRNA-mediated gene interference targeted to the env gene (NL4-3: 7490-7508) in HIV-1 infected cells, we constructed tandem-type and hairpin-type siRNA expression vectors, which were under the control of two U6 promoters. We also constructed lentiviral-based siRNA expression vectors for further assessment of their antiviral activity in transduced cells. At both the transient plasmid and lentiviral-mediated RNA expression levels, the siRNA encoding the env fragment exhibited sequence-specific suppression of target gene expression and strongly inhibited (≥90%) HIV-1 infection in the cells, as compared to the antisense RNA expression vector. Targeting the HIV-1 env gene with siRNAs encoding the env gene fragment (7490–7508) might be an effective strategy for gene therapy applications in HIV-1/AIDS treatment and management. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RNA
*GENE therapy
*MESSENGER RNA
*HIV infections
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 15257770
- Volume :
- 25
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Nucleosides, Nucleotides & Nucleic Acids
- Publication Type :
- Academic Journal
- Accession number :
- 21807364
- Full Text :
- https://doi.org/10.1080/15257770600726083