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Identification of tocopherol-associated protein as an activin/TGF-β-inducible gene in mast cells
- Source :
-
BBA - Molecular Cell Research . Aug2006, Vol. 1763 Issue 8, p900-906. 7p. - Publication Year :
- 2006
-
Abstract
- Abstract: Previous studies have demonstrated that treatment with activin A and TGF-β1, members of the TGF-β family, stimulated maturation of mouse bone marrow-derived cultured mast cells (BMMC), which was characterized by morphology and gene expression of mouse mast cell proteases (mmcps). In order to gain a better understanding of activin A- and TGF-β1-induced maturation in mast cells, we investigated the genes that were up-regulated in response to treatment with these two members of the TGF-β family. The cDNA microarray analyses indicated that in BMMC, five genes were induced by treatment with 4 nM activin A for 2 h. Tocopherol-associated protein (Tap) was one of the induced genes, and the Tap induction in response to activin A treatment was confirmed by real-time RT-PCR analyses. Treatment with TGF-β1 at 200 pM but not BMP-2 at 4 nM also increased Tap gene transcript in BMMC. Activin A-induced Tap expression was detected in BMMC but not in RAW264 macrophage-like cells, B16 melanoma cells or P19 embryonic carcinoma cells. Treatment with >1 μM SB431542, an inhibitor of activin and TGF-β type I receptors ALK4/5, reduced responsiveness of Tap expression to TGF-β1, whereas <0.5 μM SB431542 effectively reduced TGF-β1-induced expression of mmcp-1 and mmcp-7. These results suggest that inhibitory effects of SB431542 are different between TGF-β-induced genes. Reporter assays indicated that Tap expression enhances transcription mediated by the activin/TGF-β pathway. Thus, the present results suggest that Tap induction in response to activin/TGF-β occurs predominantly in mast cells and serves as a positive regulator in activin/TGF-β signaling. [Copyright &y& Elsevier]
- Subjects :
- *PEPTIDE hormones
*TRANSFORMING growth factors-beta
*GLYCOPROTEINS
*BASOPHILS
Subjects
Details
- Language :
- English
- ISSN :
- 01674889
- Volume :
- 1763
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- BBA - Molecular Cell Research
- Publication Type :
- Academic Journal
- Accession number :
- 22010256
- Full Text :
- https://doi.org/10.1016/j.bbamcr.2006.06.002