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A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energy ion beam irradiation.
- Source :
-
Applied Microbiology & Biotechnology . Aug2006, Vol. 72 Issue 3, p456-461. 6p. 1 Diagram, 1 Chart, 3 Graphs. - Publication Year :
- 2006
-
Abstract
- Agrobacterium tumefaciens ATCC4452 cells were irradiated by nitrogen ion beam, a new mutagen, with energy of 10 keV and fluence ranging from 2.6×1014 ions/cm2 to 6.5×1015 ions/cm2. A similar “saddle shape” survival curve due to ion beam irradiation appeared again in this study. Some mutants with high yield of ubiquinone-10 were induced by ion implantation. High mutation rate and wide mutation spectrum were also observed in the experiment. These results suggested that the mutagenic effect of such low-energy ion influx into bacterium cells could result from multiple processes involving direct collision of particles with cytoplasm, nucleolus, and cascade atomic and molecular reactions due to plentiful primary and secondary particles. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AGROBACTERIUM tumefaciens
*AGROBACTERIUM
*IONS
*MUTAGENS
*BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 72
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 22028952
- Full Text :
- https://doi.org/10.1007/s00253-005-0283-4