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Liver carboxylesterase cleaves surfactant protein (SP-) B and promotes surfactant subtype conversion
- Source :
-
Biochemical & Biophysical Research Communications . Oct2006, Vol. 348 Issue 4, p1449-1454. 6p. - Publication Year :
- 2006
-
Abstract
- Abstract: Conversion of the biophysically active large surfactant aggregate subtype of alveolar surfactant into the less surface active small surfactant aggregates occurs in vitro and in vivo, possibly in dependency of a carboxylesterase, entitled surfactant convertase. The substrate has yet not been safely identified. Utilizing the in vitro cycling assay we investigated conversion of an organic rabbit lavage extract reconstituted with SP-A. Porcine liver carboxylesterase, which is closely related to surfactant convertase, induced subtype conversion to a similar degree as compared with native lavage fluid containing endogenous convertase. In addition, we asked for cleavage products of SP-B and identified a ∼12kDa band upon cycling with liver carboxylesterase, having the same N-terminus as mature SP-B. A band of same molecular weight was found in native lavage fluid after in vitro conversion mediated by the endogenous convertase. We conclude that SP-B plays a pivotal role during subtype conversion and represents the substrate for surfactant convertase. [Copyright &y& Elsevier]
- Subjects :
- *BILIARY tract
*IRRIGATION (Medicine)
*ABDOMEN
*MOLECULAR weights
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 348
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 22075666
- Full Text :
- https://doi.org/10.1016/j.bbrc.2006.08.013