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Structural characterisation of novel lichen heteroglycans by NMR spectroscopy and methylation analysis
- Source :
-
Carbohydrate Research . Oct2006, Vol. 341 Issue 14, p2449-2455. 7p. - Publication Year :
- 2006
-
Abstract
- Abstract: Two galactofuranomannans, Ths-4 and Ths-5, were isolated from the lichen, Thamnolia vermicularis var. subuliformis, using ethanol fractionation and anion-exchange and size-exclusion chromatography. The average molecular weights of Ths-4 and Ths-5 were estimated to be 19 and 200kDa, respectively. Structural characterisation of Ths-4, Ths-5 and their partially hydrolysed derivatives was performed by methanolysis and methylation analysis. The intact and partially hydrolysed Ths-4 was further analysed using NMR spectroscopy (1D, COSY, NOESY, TOCSY, HSQC and HMBC). According to the data obtained, the heteroglycans Ths-4 and Ths-5 have similar structures, but have large differences in molecular weight. The structure is composed of 3-O-linked and 5-O-linked galactofuranosyl chains linked to a mannan core. The mannan core consists of a main chain of α-(1→6)-linked mannopyranosyl residues, substituted at O-2 with either a single α-mannopyranosyl unit or an α-Manp-(1→2)-α-Manp-(1→2)-α-Manp group in the ratio of approximately 1:3, respectively. The polysaccharides have idealised repeating blocks as is shown. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00086215
- Volume :
- 341
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Research
- Publication Type :
- Academic Journal
- Accession number :
- 22134179
- Full Text :
- https://doi.org/10.1016/j.carres.2006.06.026