Effect of repetitive ischemic preconditioning on spinal cord ischemia in a rabbit model

Abstract: A completely randomized controlled study based on a rabbit model was designed to study the effect of repetitive ischemic preconditioning (IPC) on a spinal cord ischemic reperfusion injury. Twenty four white adult Japanese rabbits were randomly assigned to one of the 3 groups (n =8 per group): Group I: sham-operation group, Group II: ischemic reperfusion group, and, Group III: IPC group. Spinal cord ischemia was induced by infra-renal aortic cross-clamp for 45 min in Group II. Before 45 min ischemia, the rabbits in Group III underwent four cycles of IPC (5 min of ischemia followed by 5 min of reperfusion). Post-operative neurological function, electromyography (EMG) of rear limbs, and spinal cord histopathological changes were measured. The concentrations of calcium, magnesium, copper, and zinc in spinal cord were measured in the 7th day. The neurological function and histopathological changes in Group II were significantly different from those in Group I or Group III (P <0.05 or 0.01). There was a more significant change of EMG in Group II than that in Group III (P <0.05). The concentrations of calcium and copper in Group II were significantly higher (P <0.05 or 0.01), but magnesium and zinc were significantly lower (P <0.05) than those in Group I. Calcium and copper in Group II were significantly higher (P <0.05), but zinc was significantly lower (P <0.01) than those in Group III. In conclusion, repetitive IPC can protect rabbit spinal cord from ischemic reperfusion injury in a timely manner, which is associated with corrections of imbalance of calcium, magnesium, copper, and zinc in the ischemic region. [Copyright &y& Elsevier]