Back to Search
Start Over
Cholinergic regulation of fuel-induced hormone secretion and respiration of SUR1-/- mouse islets.
- Source :
-
American Journal of Physiology: Endocrinology & Metabolism . Sep2006, Vol. 291, pE525-E535. 11p. 1 Diagram, 7 Graphs. - Publication Year :
- 2006
-
Abstract
- Neural and endocrine factors (i.e., Ach and GLP-1) restore defective glucose-stimulated insulin release in pancreatic islets lacking sulfonylurea type 1 receptors (SUR1-/-) (Doliba NM, Qin w, Vatamaniuk MZ, Li C, Zelent D, Najafi H, Buettger CW, Collins HW, Carr RD, Magnuson MA, and Matschinsky FM. Am J Physiol Endocrinol Metab 286: E834–E843, 2004). The goal of the present study was to assess fuel-induced respiration in SUR1-/- islets and to correlate it with changes in intracellular Ca2+, insulin, and glucagon secretion. By use of a method based on O2 quenching of phosphorescence, the O2 consumption rate (OCR) of isolated islets was measured online in a perifusion system. Basal insulin release (IR) was 7–10 times higher in SUR1-/- compared with control (CON) islets, but the OCR was comparable. The effect of high glucose (16.7 mM) on IR and OCR was markedly reduced in SUR1-/- islets compared with CON. Ach (0.5 μM) in the presence of 16.7 mM glucose caused a large burst of IR in CON and SUR1-/- islets with minor changes in OCR in both groups of islets. In SUR1-/- islets, high glucose failed to inhibit glucagon secretion during stimulation with amino acids or Ach. We conclude that 1) reduced glucose responsiveness of SUR1-/- islets may be in part due to impaired energetics, as evidenced by significant decrease in glucose-stimulated OCR; 2) elevated intracellular Ca2+ levels may contribute to altered insulin and glucagon secretion in SUR1-/- islets; and 3) The amplitudes of the changes in OCR during glucose and Ach stimulation do not correlate with IR in normal and SUR1-/- islets suggesting that the energy requirements for exocytosis are minor compared with other ATP-consuming reactions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ACETYLCHOLINE
*INSULIN synthesis
*GLUCAGON
*CALCIUM
*OXYGEN
*ISLANDS of Langerhans
Subjects
Details
- Language :
- English
- ISSN :
- 01931849
- Volume :
- 291
- Database :
- Academic Search Index
- Journal :
- American Journal of Physiology: Endocrinology & Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 22186353
- Full Text :
- https://doi.org/10.1152/ajpendo.00579.2005