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FRET analysis reveals a critical conformational change within the Na,K-ATPase α1 subunit N-terminus during GPCR-dependent endocytosis
- Source :
-
FEBS Letters . Sep2006, Vol. 580 Issue 21, p5067-5070. 4p. - Publication Year :
- 2006
-
Abstract
- Abstract: Dopamine is a major regulator of sodium reabsorption in proximal tubule epithelia. It induces the endocytosis of plasma membrane Na,K-ATPase molecules, and this results in a reduced capacity of the cells to transport sodium. Dopamine induces the phosphorylation of Ser-18 in the α1-subunit of Na,K-ATPase. Fluorescence resonance energy transfer analysis of cells expressing YFP-α1 and β1-CFP reveals that treatment of the cells with dopamine increases energy transfer between CFP and YFP. This is consistent with a protein conformational change that results in the N-terminal end of α1 moving closer to the internal face of the plasma membrane. [Copyright &y& Elsevier]
- Subjects :
- *DOPAMINE
*CATECHOLAMINES
*ADENOSINE triphosphatase
*FLUORESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 580
- Issue :
- 21
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 22281310
- Full Text :
- https://doi.org/10.1016/j.febslet.2006.08.035