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Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis
- Source :
-
Journal of Microbiological Methods . Nov2006, Vol. 67 Issue 2, p330-338. 9p. - Publication Year :
- 2006
-
Abstract
- Abstract: Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis. [Copyright &y& Elsevier]
- Subjects :
- *GEL electrophoresis
*ELECTROPHORESIS
*PUBLIC health
*NEISSERIA meningitidis
Subjects
Details
- Language :
- English
- ISSN :
- 01677012
- Volume :
- 67
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Microbiological Methods
- Publication Type :
- Academic Journal
- Accession number :
- 22472159
- Full Text :
- https://doi.org/10.1016/j.mimet.2006.04.004