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Proteomic analysis for protein carbonyl as an indicator of oxidative damage in senescence-accelerated mice
- Source :
-
Free Radical Research . Nov2006, Vol. 40 Issue 11, p1173-1181. 8p. 4 Diagrams, 1 Chart, 2 Graphs. - Publication Year :
- 2006
-
Abstract
- The senescence-accelerated prone mouse strain 8 (SAMP8) exhibits a remarkable age-accelerated deterioration in learning and memory. In this study, we identified carbonyl modification, a marker of protein oxidation, in liver and brain of SAMP8 from peptide mass fingerprints using MALDI-TOF mass spectrometry in combination with LC-MS/MS analysis. Carbonyl modification of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) in liver at 3 month and hippocampal cholinergic neurostimulating peptide precursor protein (HCNP-pp) in brain at 9 month were higher in SAMP8 compared with control SAMR1. We demonstrated carbonyl modification of purified Cu,Zn-SOD increased by the reaction with H2O2. Therefore, progressive accumulation of oxidative damage to Cu,Zn-SOD, may cause dysfunction of defense systems against oxidative stress in SAMP8 with a higher oxidative states, leading to acceleration of aging. Furthermore, carbonyl modification of HCNP-pp may be involved in pathophysiological alterations associated with deterioration in the learning and memory in the brain seen in SAMP8. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEOMICS
*CARBONYL compounds
*OXIDATION
*SPECTROMETRY
*AGING
Subjects
Details
- Language :
- English
- ISSN :
- 10715762
- Volume :
- 40
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Free Radical Research
- Publication Type :
- Academic Journal
- Accession number :
- 22564353
- Full Text :
- https://doi.org/10.1080/10715760600847580