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The anti-apoptotic livin gene is an important determinant for the apoptotic resistance of non-small cell lung cancer cells
- Source :
-
Lung Cancer (01695002) . Nov2006, Vol. 54 Issue 2, p135-142. 8p. - Publication Year :
- 2006
-
Abstract
- Summary: Cancer cells are typically characterized by increased resistance towards apoptosis. Livin (alternatively called ML-IAP or KIAP) is an anti-apoptotic protein which is expressed in several cancer forms. Using real-time reverse transcription-polymerase chain reaction (RT-PCR), we confirmed livin expression in a significant portion of non-small cell lung cancer (NSCLC) tissue samples and, in addition, detected livin expression in a number of NSCLC cell lines. In order to elucidate whether livin contributes to the apoptotic resistance of lung cancer cells, we silenced endogenous livin expression in a panel of cancer-derived NSCLC cell lines by RNA interference (RNAi). We observed that the targeted inhibition of livin strongly sensitized NSCLC cells to different pro-apoptotic stimuli, such as UV-irradiation or the chemotherapeutic drug etoposide. In addition, long-term silencing of livin blocked the outgrowth of NSCLC cells in colony formation assays. These effects of small interfering (si)RNA were specific for livin-expressing tumor cells. Our results indicate that Livin is an important contributor to the apoptosis resistance of NSCLC cells and may serve as a novel molecular target for therapeutic inhibition in NSCLC. [Copyright &y& Elsevier]
- Subjects :
- *CANCER cells
*APOPTOSIS
*CELL death
*CELLULAR pathology
Subjects
Details
- Language :
- English
- ISSN :
- 01695002
- Volume :
- 54
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Lung Cancer (01695002)
- Publication Type :
- Academic Journal
- Accession number :
- 22580348
- Full Text :
- https://doi.org/10.1016/j.lungcan.2006.07.018