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Subproteomic study of hepatitis C virus replicon reveals Ras-GTPase-activating protein binding protein 1 as potential HCV RC component

Authors :
Yi, Zhigang
Fang, Caiyun
Pan, Tingting
Wang, Jiadong
Yang, Pengyuan
Yuan, Zhenghong
Source :
Biochemical & Biophysical Research Communications. Nov2006, Vol. 350 Issue 1, p174-178. 5p.
Publication Year :
2006

Abstract

Abstract: Hepatitis C virus (HCV) RNA synthesis takes place on a detergent resistant membrane (DRM) structure. To identify potential cellular proteins related to HCV replication complexes (RC), we purified DRMs from HCV subgenomic replicon cells and its parental Huh7 cells. The proteins of DRM fractions were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Comparing with parental Huh7 cells, 60 proteins were up-regulated while 14 proteins were down-regulated in HCV replicon cells. Ras-GTPase-activating protein binding protein 1 (G3BP1), one of the elevated proteins, was found to be associated with HCV NS5B and knockdown of G3BP1 by siRNA in HCV replicon cells significantly reduced HCV replication, which may indicate it a potential component of HCV RC. These results suggest that HCV viral gene and proteins may regulate the presence of host cellular proteins in DRM, ensure appropriate concentrations of replication components, and hence control the rates or efficiencies of HCV replication. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
0006291X
Volume :
350
Issue :
1
Database :
Academic Search Index
Journal :
Biochemical & Biophysical Research Communications
Publication Type :
Academic Journal
Accession number :
22594143
Full Text :
https://doi.org/10.1016/j.bbrc.2006.09.027