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Characterization of the second external alternative dehydrogenase from mitochondria of the respiratory yeast Kluyveromyces lactis

Authors :
Tarrío, Nuria
Cerdán, M. Esperanza
Siso, M. Isabel González
Source :
BBA - Bioenergetics. Nov2006, Vol. 1757 Issue 11, p1476-1484. 9p.
Publication Year :
2006

Abstract

Abstract: The mitochondria of the respiratory yeast Kluyveromyces lactis are able to reoxidize cytosolic NADPH. Previously, we characterized an external alternative dehydrogenase, KlNde1p, having this activity. We now characterize the second external alternative dehydrogenase of K. lactis mitochondria, KlNde2p. We examined its role in cytosolic NADPH reoxidation by studying heterologous expression of KlNDE2 in Saccharomyces cerevisiae mutants and by constructing Δklnde1 and Δklnde2 mutants. KlNde2p uses NADH or NADPH as substrates, its activity in isolated mitochondria is not regulated by exogenously added calcium and it is not down-regulated when the cells grow in glucose versus lactate. KlNde2p shows lower affinity for NADPH than KlNde1p. Both enzymes show similar pH optimum. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00052728
Volume :
1757
Issue :
11
Database :
Academic Search Index
Journal :
BBA - Bioenergetics
Publication Type :
Academic Journal
Accession number :
22964960
Full Text :
https://doi.org/10.1016/j.bbabio.2006.09.002