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Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps.
- Source :
-
Allergy . Jan2007, Vol. 62 Issue 1, p66-72. 7p. 1 Color Photograph, 1 Chart, 4 Graphs. - Publication Year :
- 2007
-
Abstract
- Background: Nasal polyps (NP), a subgroup of chronic rhinosinusitis, are characterized by interleukin 5 (IL-5) mediated infiltration of eosinophils in sinus mucosa, leading to pseudostratified ciliated columnar epithelium, thickening of the epithelial basement membrane and tissue edema. Matrix metalloproteinases (MMP) constitute a large group of Zn2+ dependent endopeptidases with the ability to degrade extracellular matrix and are possibly responsible for the development of tissue edema in chronic sinusitis. Objective: The aim of this study was to determine the expression of MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA and to locate the distribution of MMP-2, MMP-9 and TIMP-1 by immunohistochemistry in ethmoid sinus mucosa in NP. Furthermore the correlation between IL-5 or IL-8 and MMP-2, MMP-9 or TIMP-1 is examined. Methods: Nasal polyps of 33 patients and 18 specimens of inferior turbinate mucosa were examined by real time RT-PCR for MMP-2, MMP-9, TIMP-1, IL-5 and IL-8 mRNA expression. Immunohistochemical labeling for MMP-2, MMP-9 and TIMP-1 was performed. Results: Differences between both locations were detectable for MMP-9 ( P < 0.001) and IL-5 ( P = 0.003) but not for MMP-2 ( P = 0.278), TIMP-1 ( P = 0.515) and IL-8 ( P = 0.386). Correlation was detected only between TIMP-1 and IL-5 ( r = 0.422, P = 0.014). Cytoplasmic staining of MMP-2 was present in the apical part of the ciliated cells, submucosal glands and in smooth muscle cells. Matrix metalloproteinase-9 was expressed in surface epithelium, in seromucous glands and in polymorphonuclear cells. Conclusions: Expression of MMP-9 and IL-5 mRNA are associated with NP. The correlation between IL-5 and TIMP-1 indicates the role of TIMP-1 in maintaining the homeostasis in NP. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 62
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 23408174
- Full Text :
- https://doi.org/10.1111/j.1398-9995.2006.01255.x