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Exploration of triple-helical fragments: crystallization and preliminary X-ray diffraction of d(TGGCCTTAAGG).
- Source :
-
Acta Crystallographica: Section F (Wiley-Blackwell) . Jan2007, Vol. 63 Issue 1, p52-55. 4p. 2 Black and White Photographs, 1 Diagram, 1 Chart. - Publication Year :
- 2007
-
Abstract
- The nonamer d(GCGAATTCG) and decamer d(GGCCAATTGG), containing one and two overhanging guanines, respectively, form G·GC triplets in their crystal packing. In order to introduce a third subsequent T·AT triplet, the decamer was further extended by one overhanging thymine residue. Two different crystal morphologies of the sequence d(TGGCCTTAAGG) were obtained by hanging-drop vapour diffusion and diffracted to 2.5 and 2.3 Å resolution, respectively. However, both crystals belong to the orthorhombic space group P212121, with similar unit-cell parameters. Therefore, the two data sets could be merged to a resolution of 2.4 Å with unit-cell parameters a = 26.97, b = 41.12, c = 52.72 Å. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRYSTAL growth
*CRYSTALS
*X-ray diffraction
*DIFFUSION
*THYMIDINE
*MORPHOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 17443091
- Volume :
- 63
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 23591803
- Full Text :
- https://doi.org/10.1107/S1744309106054522