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Thioquinolobactin, a Pseudomonas siderophore with antifungal and anti- Pythium activity.

Authors :
Matthijs, Sandra
Tehrani, Kourosch Abbaspour
Laus, George
Jackson, Robert W.
Cooper, Richard M.
Cornelis, Pierre
Source :
Environmental Microbiology. Feb2007, Vol. 9 Issue 2, p425-434. 10p. 3 Diagrams, 3 Graphs.
Publication Year :
2007

Abstract

Under conditions of iron limitation Pseudomonas fluorescens ATCC 17400 produces two siderophores, pyoverdine, and a second siderophore quinolobactin, which itself results from the hydrolysis of the unstable molecule 8-hydroxy-4-methoxy-2-quinoline thiocarboxylic acid (thioquinolobactin). Pseudomonas fluorescens ATCC 17400 also displays a strong in vitro antagonism against the Oomycete Pythium, which is repressed by iron, suggesting the involvement of a siderophore(s). While a pyoverdine-negative mutant retains most of its antagonism, a thioquinolobactin-negative mutant only slowed-down Pythium growth, and a double pyoverdine-, thioquinolobactin-negative mutant, which does not produce any siderophore, totally lost its antagonism against Pythium. The siderophore thioquinolobactin could be purified and identified from spent medium and showed anti- Pythium activity, but it was quickly hydrolysed to quinolobactin, which we showed has no antimicrobial activity. Analysis of antagonism-affected transposon mutants revealed that genes involved in haem biosynthesis and sulfur assimilation are important for the production of thioquinolobactin and the expression of antagonism. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14622912
Volume :
9
Issue :
2
Database :
Academic Search Index
Journal :
Environmental Microbiology
Publication Type :
Academic Journal
Accession number :
23626524
Full Text :
https://doi.org/10.1111/j.1462-2920.2006.01154.x