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Panmicrobial oligonucleotide array for diagnosis of infectious diseases.
- Source :
-
Emerging Infectious Diseases . Jan2007, Vol. 13 Issue 1, p73-81. 9p. - Publication Year :
- 2007
-
Abstract
- To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COMMUNICABLE diseases
*DIFFERENTIAL diagnosis
*NUCLEOTIDES
*BACTERIA
*FUNGI
*PARASITES
*NUCLEIC acids
*COMMUNICABLE disease diagnosis
*MALARIA diagnosis
*VIRAL disease diagnosis
*COMPARATIVE studies
*DISEASE complications
*EPIDEMICS
*BIOLOGICAL evolution
*RESEARCH methodology
*MEDICAL cooperation
*RESEARCH
*RESEARCH funding
*VIRUS diseases
*EVALUATION research
*TREATMENT effectiveness
*OLIGONUCLEOTIDE arrays
Subjects
Details
- Language :
- English
- ISSN :
- 10806040
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 23757954
- Full Text :
- https://doi.org/10.3201/eid1301.060837