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Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish
- Source :
-
Aquaculture . Feb2007, Vol. 262 Issue 1, p1-9. 9p. - Publication Year :
- 2007
-
Abstract
- Abstract: Co-infection of two viruses has been observed in mandarin fish (Siniperca chuatsi), but the two viruses have not been characterized. In this study, a rhabdovirus has been isolated from the co-infected two viruses extracted from the diseased mandarin fish, and its morphological structure and partial biochemical and biophysical characteristics have been observed and analyzed. The isolated rhabdovirus has a typical bullet shape, and is therefore called S. chuatsi rhabdovirus (SCRV). And, the isolated rhabdovirus produced a higher titer (108.5 TCID50 ml−1) than did the co-infecting viruses (106.5 TCID50 ml−1). Subsequently, the viral genome RNA was extracted, and used as template to clone the complete nucleoprotein (N) gene by RT-PCR amplification. Cloning and sequencing of the SCRV N protein revealed 42%–31% amino acid identities to that of trout rhabdovirus 903/87 and the rhabdoviruses in genus Vesiculovirus. SDS-PAGE separation of the isolated SCRV and other two rhabdoviruses also revealed obvious polypeptide profile difference. Moreover, the anti-SCRV N protein antibody was prepared, and the anti-SCRV N protein antibody only could recognize the SCRV N protein, whereas no antigenicity was detected in other two rhabdoviruses. The data suggested that the SCRV should be a rhabdovirus member related to the genus Vesiculovirus in the Rhabdoviridae. [Copyright &y& Elsevier]
- Subjects :
- *RHABDOVIRUSES
*RNA viruses
*FISHES
*GENETICS
Subjects
Details
- Language :
- English
- ISSN :
- 00448486
- Volume :
- 262
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Aquaculture
- Publication Type :
- Academic Journal
- Accession number :
- 23806671
- Full Text :
- https://doi.org/10.1016/j.aquaculture.2006.09.030