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Examination of real-time PCR for HIV-1 RNA and DNA quantitation in patients infected with HIV-1 BF intersubtype recombinant variants
- Source :
-
Journal of Virological Methods . Mar2007, Vol. 140 Issue 1/2, p222-227. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: The impact of HIV-1 genetic diversity on the performance of laboratory testing is an issue that has to be monitored continuously. An “in-house” real-time PCR assay was developed by the Agence Nationale de Recherche sur le SIDA (ANRS) in France for viral load (VL) quantitation based on the amplification of the HIV-1 long terminal repeat (LTR) region. This technology has not been used in Argentina yet and considering the HIV-1 diversity in the country, a comparative analysis of this assay was undertaken versus the Versant HIV-1 RNA 3.0 Assay (b-DNA). The performance was assessed on 30 drug-naïve HIV-1 infected patients who were characterized previously by phylogenetic analysis of the pol and vpu gene. The results showed that there is a significant linear correlation between values of transformed viral load logarithms measured by both, bDNA and real-time PCR assay and that this assay can be used to quantify viral load in samples from BF-infected patients with the same accuracy and reliability as for B subtype samples. The use of “in-house” real-time PCR to measure DNA in PBMCs correlated strongly with the HIV-1 RNA levels in all specimens. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01660934
- Volume :
- 140
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Virological Methods
- Publication Type :
- Academic Journal
- Accession number :
- 23864758
- Full Text :
- https://doi.org/10.1016/j.jviromet.2006.11.012