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Direct electron transfer and electrocatalysis of hemoglobin adsorbed on mesoporous carbon through layer-by-layer assembly
- Source :
-
Biosensors & Bioelectronics . Mar2007, Vol. 22 Issue 8, p1618-1624. 7p. - Publication Year :
- 2007
-
Abstract
- Abstract: Using chitosan as an effective linker between CMK-3 and glassy carbon electrode surface, {Hb/CMK-3} n multilayer film-modified electrodes were constructed through layer-by-layer assembly. The morphology of thus-formed {Hb/CMK-3} n film was characterized by scanning electron micrographs, and the interaction of hemoglobin (Hb) with CMK-3 was studied by UV–vis spectroscopy and electrochemical methods. Under optimal conditions, {Hb/CMK-3}6 film showed a couple of stable and well-defined redox peaks at about −377 and −296mV in pH 7.0 buffers. Furthermore, the {Hb/CMK-3}6 film displayed excellent electrocatalysis to the reduction of both H2O2 and O2. Based on thus-formed film and its direct electron transfer behavior, a novel biosensor was presented for the determination of H2O2 ranging from 1.2 to 57μM with the detection limit of 0.6μM at S/N=3. CMK-3 provided a desirable matrix for protein immobilization and biosensor preparation. [Copyright &y& Elsevier]
- Subjects :
- *CARBON electrodes
*HEMOGLOBINS
*CHARGE exchange
*ELECTROCATALYSIS
Subjects
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 22
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 23866140
- Full Text :
- https://doi.org/10.1016/j.bios.2006.07.022