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Differential gene expression profiles in the hippocampus of senescence-accelerated mouse
- Source :
-
Neurobiology of Aging . Apr2007, Vol. 28 Issue 4, p497-506. 10p. - Publication Year :
- 2007
-
Abstract
- Abstract: The senescence-accelerated mouse (SAM) is an animal model for studying senescence and age-associated disorders due to its inherited aging phenotype. The SAM/prone8 (SAMP8) is a useful animal model to investigate the fundamental mechanisms involved in age-related learning and memory deficits that may have relevance to age-associated AD, while SAM/resistant1 (SAMR1) shows normal. To identify genes rendering the cognitive deterioration with aging, the subtractive cDNA libraries containing 1924 clones with the positive ratio of 96.18% were generated and the microarray containing 3136 cDNA was prepared. The results of screening libraries by the microarray showed that of all 91 differentially expressed genes, 50 were over-expressed and 41 were low-expressed in SAMP8. Some of the identified genes were confirmed by the real time quantitative RT-PCR. These results indicated the profiles of gene expression in the hippocampus of SAMP8 and SAMR1 were significantly different, which may play important roles in the age-related cognitive deficit in SAMP8, suggesting those genes related to the cognitive deficient or pathology change in the brain of SAMP8 may be potential gene targets for Alzheimer''s disease therapy. [Copyright &y& Elsevier]
- Subjects :
- *ANIMAL experimentation
*PHENOTYPES
*GENES
*PATHOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 01974580
- Volume :
- 28
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Neurobiology of Aging
- Publication Type :
- Academic Journal
- Accession number :
- 24137950
- Full Text :
- https://doi.org/10.1016/j.neurobiolaging.2006.02.004