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Analysis of Melatonin in Dosage Formulation by Capillary Electrophoresis.
- Source :
-
Journal of Liquid Chromatography & Related Technologies . 2007, Vol. 30 Issue 4, p545-556. 12p. - Publication Year :
- 2007
-
Abstract
- A simple, fast, and reproducible capillary electrophoretic method is developed and validated for analysis of melatonin in pharmaceutical preparations. The CE method was developed by using a fused silica capillary (60 cm×50 µm I.D.), phosphate buffer (50 mM, 3.0 pH) as the background electrolyte (BGE), 20 kV applied voltage with UV detection at 214 nm, and at a working temperature of 23±1°C. Linearity was observed in the concentration range from 10 to 100 µg/L with a correlation coefficient (R2) of 0.9996. The limits of detection and quantification achieved were 10 and 15 µg/mL, respectively. The percentage recovery of melatonin from pharmaceutical preparations was 95.0. Validation parameters prove the precision of the method and its applicability for the determination of melatonin in pharmaceutical tablet formulations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10826076
- Volume :
- 30
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Liquid Chromatography & Related Technologies
- Publication Type :
- Academic Journal
- Accession number :
- 24154625
- Full Text :
- https://doi.org/10.1080/10826070601093861