Induced interspecific androgenesis using diploid sperm from allotetraploid hybrids of common carp×red crucian carp

Abstract: In the present study, viable diploid androgenetic individuals (AT-ag) (2n =100) have been developed in UV-radiated eggs of goldfish (GF) (Carassius auratus) following fertilization with diploid spermatozoa from male allotetraploid hybrid (AT) F13 (4n =200) of red crucian carp (C. auratus red var.) (♀)×common carp (Cyprinus carpio L.) (♂). In the experimental groups, eggs were inactivated by UV-rays, the range of UV-radiation dose tested varied from 240 to 600 mJ/cm2, the relative high hatching rate (4.1±0.4%) and survival from hatching to start of feeding (41.3±2.6%) were given at UV dose of 300 mJ/cm2. The androgenetic nature was confirmed by chromosome observation and DNA content measurement of somatic cells by a flow cytometer. The AT-ag were diploid (2n =100) whereas the controls (GF×AT) were triploids (3n =150). The diploid AT-ag grew slower than the triploid GF×AT. The AT-ag and GF×AT were also morphologically distinguishable. The AT-ag began to sexually mature at the age of 2 years, and their fertility further increased at the age of 3 years. The tetraploids were obtained in the offspring (F1ag×ag) produced by mating the males with females of AT-ag and in the progeny (AT-ag×AT) generated by crossing the males of AT with the females of AT-ag, indicating that diploid AT-ag could produce diploid gametes. All the sex ratios of males to females in AT-ag, F1ag×ag, AT-ag×AT were not significant (P > 0.05) from the ratio of 1:1, suggesting the XXXY sex-determining mechanism in the males of AT. The present study indicated that bisexual fertile diploid AT-ag were successfully induced from diploid spermatozoa of AT. [Copyright &y& Elsevier]