Top 10 HPLC Column Myths.

In any field there are often ‘misconceptions’ or ‘myths’ that are perpetuated and passed on to the next generation. These myths are often driven by a lack of understanding by practitioners of the real issues. In this instalment of ‘Column Watch’, the 10 most popular myths around high performance liquid chromatography (HPLC) column technology will be demystified by discussing the issues at hand. Among some of the popular myths that will be dispelled are that ‘All C18 (L1) columns are the same’, ‘You can't reverse an HPLC column’, ‘High temperature always leads to better separations’ and ‘The higher the carbon load the better the reversed-phase column’. [ABSTRACT FROM AUTHOR]