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Phenolic-rich fraction from Rhus verniciflua Stokes (RVS) suppress inflammatory response via NF-κB and JNK pathway in lipopolysaccharide-induced RAW 264.7 macrophages
- Source :
-
Journal of Ethnopharmacology . Apr2007, Vol. 110 Issue 3, p490-497. 8p. - Publication Year :
- 2007
-
Abstract
- Abstract: The effects of phenolic-rich fraction (PRF) from Rhus verniciflua Stokes (Anacardiaceae) on the activities of cellular signaling molecules that mediate inflammatory responses in LPS-induced RAW 264.7 macrophages were investigated. At various concentrations of PRF significantly inhibited NO, PGE2 and TNF-α production in LPS-induced RAW 264.7 macrophage cells. The PRF also significantly inhibited iNOS and COX-2 protein expression in LPS-induced RAW 264.7 macrophage in a concentration-dependent manner. Transcription factor NF-κB plays a key role for the inducible expression of genes mediating proinflammatory effects and here, we show that PRF can inhibit the induction of NF-κB activity. The PRF effectively inhibited the iNOS and COX-2 protein expression through suppression of phospho-JNK1/2 activation. Study using PDA HPLC has found that the PRF contains several low molecular compounds (i.e. p-coumaric acid, fustin, kaempferol-3-O-glucoside, sulfuretin, butein, kaempferol). Our results indicate that the anti-inflammatory properties of PRF might result from the inhibition of pro-inflammatory mediators (e.g., NO, PGE2 and TNF-α) by suppression of such signaling pathways as NF-κB and JNK1/2. [Copyright &y& Elsevier]
- Subjects :
- *TOXICODENDRON vernicifluum
*MACROPHAGES
*NF-kappa B
*CYCLOOXYGENASE 2
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 110
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 24385771
- Full Text :
- https://doi.org/10.1016/j.jep.2006.10.013