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Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum.
- Source :
-
Acta Crystallographica: Section D (Wiley-Blackwell) . Apr2007, Vol. 63 Issue 4, p458-464. 7p. 2 Diagrams, 3 Charts. - Publication Year :
- 2007
-
Abstract
- The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 Å, from c = 81 Å to c = 97 Å. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 Å; form 2, c = 92 Å; form 3, c = 81 Å). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09074449
- Volume :
- 63
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section D (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 24458061
- Full Text :
- https://doi.org/10.1107/S0907444907003228