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The bovine seminal plasma protein PDC-109 extracts phosphorylcholine-containing lipids from the outer membrane leaflet.

Authors :
Tannert, Astrid
Kurz, Anke
Erlemann, Karl-Rudolf
Müller, Karin
Herrmann, Andreas
Schiller, Jürgen
Töpfer-Petersen, Edda
Manjunath, Puttaswamy
Müller, Peter
Source :
European Biophysics Journal. Apr2007, Vol. 36 Issue 4/5, p461-475. 15p. 7 Graphs.
Publication Year :
2007

Abstract

The bovine seminal plasma protein PDC-109 modulates the maturation of bull sperm cells by removing lipids, mainly phosphatidylcholine and cholesterol, from their cellular membrane. Here, we have characterized the process of extraction of endogenous phospholipids and of their respective analogues. By measuring the PDC-109-mediated release of fluorescent phospholipid analogues from lipid vesicles and from biological membranes (human erythrocytes, bovine epididymal sperm cells), we showed that PDC-109 extracts phospholipids with a phosphorylcholine headgroup mainly from the outer leaflet of these membranes. The ability of PDC-109 to extract endogenous phospholipids from epididymal sperm cells was followed by mass spectrometry, which allowed us to characterize the fatty acid pattern of the released lipids. From these cells, PDC-109 extracted phosphatidylcholine and sphingomyelin that contained an enrichment of mono- and di-unsaturated fatty acids as well as short-chain and lyso-phosphatidylcholine species. Based on the results, a model explaining the phospholipid specificity of PDC-109-mediated lipid release is presented. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01757571
Volume :
36
Issue :
4/5
Database :
Academic Search Index
Journal :
European Biophysics Journal
Publication Type :
Academic Journal
Accession number :
24731938
Full Text :
https://doi.org/10.1007/s00249-006-0105-3