Back to Search
Start Over
Model for T-Antigen-Dependent Melting of the Simian Virus 40 Core Origin Based on Studies of the Interaction of the Beta-Hairpin with DNA.
- Source :
-
Journal of Virology . May2007, Vol. 81 Issue 9, p13-13. 1p. - Publication Year :
- 2007
-
Abstract
- The interaction of simian virus 40 (SV40) T antigen (T-ag) with the viral origin has served as a model for studies of site-specific recognition of a eukaryotic replication origin and the mechanism of DNA unwinding. These studies have revealed that a motif termed the "beta-hairpin" is necessary for assembly of T-ag on the SV40 origin. Herein it is demonstrated that residues at the tip of the "beta-hairpin" are needed to melt the origin-flanking regions and that the T-ag helicase domain selectively assembles around one of the newly generated single strands in a manner that accounts for its 3′-to-5′ helicase activity. Furthermore, T-ags mutated at the tip of the "beta-hairpin" are defective for oligomerization on duplex DNA; however, they can assemble on hybrid duplex DNA or single-stranded DNA (ssDNA) substrates provided the strand containing the 3′ extension is present. Collectively, these experiments indicate that residues at the tip of the beta-hairpin generate ssDNA in the core origin and that the ssDNA is essential for subsequent oligomerization events. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SIMIAN viruses
*VIRAL replication
*ANTIGENS
*DNA helicases
*ISOMERASES
Subjects
Details
- Language :
- English
- ISSN :
- 0022538X
- Volume :
- 81
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 24856205
- Full Text :
- https://doi.org/10.1128/JVI.02451-06