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Preparation of low-molecular-weight polyguluronate sulfate and its anticoagulant and anti-inflammatory activities
- Source :
-
Carbohydrate Polymers . Jun2007, Vol. 69 Issue 2, p272-279. 8p. - Publication Year :
- 2007
-
Abstract
- Low-molecular-weight polyguluronate sulfate (LPGS) was prepared as a heparinoid by chemical sulfation of polyguluronic acid (PG), which was isolated from the hydrolysate of alginate by pH-fractionated precipitation. The structure of LPGS was elucidated based on FT-IR and 2D NMR spectroscopy. Sulfation was demonstrated to occur at the C-2 and C-3 positions of the guluronic acid residues and the degree of substitution (DS) was 1.53 per monosaccharide residue. The weight-averaged molecular weight of LPGS was 11.4kDa determined by high performance gel permeation chromatography (HPGPC). The in vitro anticoagulant activity and in vivo anti-inflammatory activity of LPGS were evaluated. Results showed that LPGS significantly prolonged the whole blood clotting time and activated partial thrombinplastin time (APTT), and exhibited considerable anti-inflammatory activity in cotton pellet-induced granuloma in rats. [Copyright &y& Elsevier]
- Subjects :
- *ANTICOAGULANTS
*ANTI-inflammatory agents
*GRANULOMA
*MEDICAL research
*POLYMERS
Subjects
Details
- Language :
- English
- ISSN :
- 01448617
- Volume :
- 69
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Polymers
- Publication Type :
- Academic Journal
- Accession number :
- 24864826
- Full Text :
- https://doi.org/10.1016/j.carbpol.2006.10.024