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Purification of deoxynivalenol from Fusarium graminearum rice culture and mouldy corn by high-speed counter-current chromatography
- Source :
-
Journal of Chromatography A . Jun2007, Vol. 1151 Issue 1/2, p187-192. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: Deoxynivalenol (DON) is a mycotoxin produced by several Fusarium species and is toxic to a wide range of organisms, including human beings and livestock. To produce large amounts of pure DON for research purposes, a novel method using high-speed counter-current chromatography (HSCCC) was developed. Rice cultured with Fusarium graminearum and field mouldy corn infected by F. graminearum were extracted with methanol and found to contain 1.16 and 1.30mg DON/g, respectively. The extracts were concentrated and then separated using a biphasic solvent system consisting of ethyl acetate–water (1:1, v/v). Collected fractions were analyzed by high-performance liquid chromatography (HPLC) and identified by congruent retention time and UV/vis spectrum and mass spectrometric data. Fractions containing DON were combined and freeze-dried. This method produced 116mg and 65mg DON with a purity of greater than 94.9% from 200g of the rice culture and the mouldy corn, respectively. The HSCCC method had a recovery rate of DON at 88% from the crude extracts of both samples. This one-step purification method provided a simple and effective tool for obtaining a large amount of DON, an essential material for studies related to toxicology and detoxification of this mycotoxin. [Copyright &y& Elsevier]
- Subjects :
- *MYCOTOXINS
*FUSARIUM
*CROPS
*COUNTERCURRENT chromatography
Subjects
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1151
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 24970455
- Full Text :
- https://doi.org/10.1016/j.chroma.2007.01.112