Back to Search
Start Over
Differential protein expression in pancreatic islets after treatment with an imidazoline compound.
- Source :
-
Cellular & Molecular Life Sciences . May2007, Vol. 64 Issue 10, p1310-1316. 7p. 2 Diagrams. - Publication Year :
- 2007
-
Abstract
- The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1). [ABSTRACT FROM AUTHOR]
- Subjects :
- *IMIDAZOLINES
*PROTEINS
*INSULIN
*GLUCOSE
*DIABETES
*THERAPEUTICS
*GEL electrophoresis
Subjects
Details
- Language :
- English
- ISSN :
- 1420682X
- Volume :
- 64
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Cellular & Molecular Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 25115694
- Full Text :
- https://doi.org/10.1007/s00018-007-7136-5