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Upregulated expression of postsynaptic density-93 and N-methyl-d-aspartate receptors subunits 2B mRNA in temporal lobe tissue of epilepsy
- Source :
-
Biochemical & Biophysical Research Communications . Jul2007, Vol. 358 Issue 3, p825-830. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: Objective: To investigate the expression of PSD-93 mRNA and NR2B mRNA in the brain tissue from the patients with epilepsy so as to explore the possible mechanisms of the pathogenesis of the epilepsy. Methods: Fifty-six patients with epilepsy were divided into intractable epilepsy (IE) and non-intractable epilepsy (NIE) groups. cDNA microarrays prepared from the brain tissues obtained from these two groups were scanned and comparison to those from the non-epileptogenic control (C) was made. Expression level of PSD-93mRNA and NR2BmRNA were examined by reverse transcription polymerase chain reaction (GAPDH gene, internal control). Expression ratio (target gene/GAPDH) was used to evaluate each gene relative expression level. Results: The cDNA microarray analysis showed that the expression of PSD-93 mRNA related to the function of NMDAR-NO signal transduction pathway was significantly higher in epilepsy patients than those in the controlled group. The results of RT-PCR were consistent with those of the cDNA microarrays. The relative expression ratio of PSD-93 in patients with non-epileptogenic control, NIE, and IE was 0.159, 0.368, and 0.341, respectively. Correspondingly, that of NR2B was 0.198, 0.738, and 0.903, respectively. The expressions of PSD-93 and NR2B in the NIE and IE were significantly higher than those of control, respectively (P <0.05). However, there was no significantly difference the expression of PSD-93 between NIE and IE. (P >0.05), neither do that of NR2B (P >0.05). Conclusions: The upregulated expressions of PSD-93 mRNA and NR2BmRNA may be involved in the pathogenesis of epilepsy. [Copyright &y& Elsevier]
- Subjects :
- *EPILEPSY
*TEMPORAL lobe
*MESSENGER RNA
*BRAIN diseases
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 358
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 25182501
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.05.010