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XPS investigation of DNA binding to zirconium-phosphonate surfaces
- Source :
-
Colloids & Surfaces B: Biointerfaces . Jul2007, Vol. 58 Issue 1, p34-38. 5p. - Publication Year :
- 2007
-
Abstract
- Abstract: The surface coverage of phosphorylated oligonucleotides immobilized on a zirconium-phosphonate surface was analyzed using X-ray photoelectron spectroscopy (XPS). By quantifying the intensity of the N 1s signal originating from the oligonucleotide and the Zr 3d peak from the metal-phosphonate surface, the surface coverage of the oligonucleotide could be calculated with a modified substrate-overlayer model. We found relatively low surface coverages indicating that once covalently bound via the terminal phosphate the polymer chain further physisorbs to the surface limiting the adsorption of additional molecules. [Copyright &y& Elsevier]
- Subjects :
- *NUCLEIC acids
*MONOMOLECULAR films
*OLIGONUCLEOTIDES
*ELECTRON spectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 09277765
- Volume :
- 58
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Colloids & Surfaces B: Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 25185512
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2006.12.015