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Characterization of two temperature-inducible promoters newly isolated from B. subtilis
- Source :
-
Biochemical & Biophysical Research Communications . Jul2007, Vol. 358 Issue 4, p1148-1153. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of β-galactosidase driven by these promoters was much higher at 45°C than that at 37°C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45°C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis. [Copyright &y& Elsevier]
- Subjects :
- *BACILLUS (Bacteria)
*ESCHERICHIA
*ESCHERICHIA coli
*ENTEROBACTERIACEAE
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 358
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 25255236
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.05.064