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NF-κB inhibition by an adenovirus expressed aptamer sensitizes TNFα-induced apoptosis
- Source :
-
Biochemical & Biophysical Research Communications . Aug2007, Vol. 359 Issue 3, p475-480. 6p. - Publication Year :
- 2007
-
Abstract
- Abstract: Prolonged activation of NF-κB is involved in the pathogenesis of chronic inflammatory diseases and associated cancers. NF-κB activation is considered to be a main mechanism opposing TNFα-induced apoptosis. We investigated whether inhibition of NF-κB could sensitize tumor and endothelial cells to TNFα-induced apoptosis. As such, we developed a novel H1 RNA polymerase III promoter driven adenoviral vector to express an RNA aptamer, Ad-A-p50, which selectively inhibits NF-κB activation in the nucleus. This event sensitizes human lung adenocarcinoma cells (A549) and human endothelial cells (HUVEC) to TNFα-induced apoptosis through the multiple pathways regulated by NF-κB, including Bcl-XL, HIF-1α, and VEGF. Our findings also suggest a new mechanism of HIF-1α regulation by NF-κB in the normoxic environment. RNA aptamer inhibition of NF-κB offers exciting opportunities for sensitizing inflammatory and tumor cells to TNFα-induced apoptosis. [Copyright &y& Elsevier]
- Subjects :
- *COMPLEMENT inhibition
*APOPTOSIS
*RNA polymerases
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 359
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 25407267
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.05.125