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Intimin subtyping of Escherichia coli: concomitant carriage of multiple intimin subtypes from forage-fed cattle and sheep.
- Source :
-
FEMS Microbiology Letters . Jul2007, Vol. 272 Issue 2, p163-171. 9p. 1 Black and White Photograph, 1 Diagram, 5 Charts. - Publication Year :
- 2007
-
Abstract
- The outer membrane protein, intimin ( eae), which mediates bacterial attachment to epithelial cells, is associated with enteropathogenic Escherichia coli and some Shiga toxin-producing E. coli. The eae subtype of E. coli strains isolated from healthy cattle and sheep was identified using a rapid PCR-restriction fragment length polymorphism (RFLP) method to produce profiles that were compared with those generated in silico. The 139 eae-positive E. coli strains were separated into 11 different PCR-RFLP profiles. The most common eae PCR-RFLP type was β (23.7%), followed by ζ (20.1%), θ (16.5%), ι (12.2%), κ (8.6%), ℇ (7.2%), γ (2.9%), ν and β2 (2.2%) and ι2 (1.4%). Four isolates did not yield a PCR-RFLP amplification product but complete sequencing of the eae gene matched subtype ρ. Two different eae variants were isolated from the same swab from 18 different animals and subtype ι was the most ‘promiscuous’, being isolated with four other eae subtypes from seven separate animals. None of the eae-positive STEC were subtype γ, which is associated with STEC serogroup O157. This method allowed the rapid identification of eae subtypes and indicates that forage-fed animals possessed a wide diversity of bacterial eae subtypes with a low frequency of eae subtype γ. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03781097
- Volume :
- 272
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- FEMS Microbiology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 25427144
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2007.00755.x