Back to Search Start Over

EXPRESSION AND FUNCTION OF UTROPHIN ASSOCIATED PROTEIN COMPLEX IN STRETCHED HUVECs; DISSOCIATION AND ACTIVATION OF eNOS.

Authors :
Ramirez-Sanchez, Israel
Ceballos-Reyes, Guillermo
Rosas-Vargas, Haydee
Cerecedo-Mercado, Doris
Zentella-Dahesa, Alejandro
Coral-Vazquez, Ramon-Mauricio
Source :
FASEB Journal. Apr2007, Vol. 21 Issue 6, pA861-A861. 1/5p.
Publication Year :
2007

Abstract

Several studies have emphasized the importance, of dystrophin-associated protein complex (DAPC) to maintain the vascular function in development of dilated cardiomyopathy in dystrophyc patients has been shown. Previously we postulated the presence of an utrophin associated protein complex (UAPC) in endothelium from umbilical cord vessels. In this work, we demonstrate that utrophin indeed forms a complex, with beta-dystroglycan, epsilon sarcoglycan, caveolin-1, and endothelial nitric oxide synthase (eNOS) in human umbilical vein endothelial cells, by co-immunoprecipitation and Western blot analysis. Additionally, we observed an increment in the levels of all proteins of complex after mechanical stretching. Interestingly, this stimulus also induced eNOS up-regulation, activation and release from the UAPC and led to a significant increase in nitric oxide production. Finally, we propose that UAPC in HUVECs may play an important role in the regulation of vascular tone during mechanical stress. CONACyT 39542-Q, 144593 [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08926638
Volume :
21
Issue :
6
Database :
Academic Search Index
Journal :
FASEB Journal
Publication Type :
Academic Journal
Accession number :
25597595