Sampling Time is Crucial for Measurement of Aerobic Exercise-Induced Oxidative Stress.

The article investigates the time-course changes of several commonly used makers of oxidative stress by performing serial measurements during a 24-hour period after an acute bout of strenuous cardiovascular exercise. The results of the study indicate that there is no best time point applying to all makers for collecting blood samples after aerobic exercise. The optimum postexercise time points for blood collection in untrained individuals are immediately after exercise for catalase, 1 hour for thiobarbituric acid-reactive substances, 2 hours for total antioxidant capacity, reduced glutathione and oxidized glutathione while 4 hours after exercise for protein carbonyls.