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Molecular “Wiring” Glucose Oxidase in Supramolecular Architecture.
- Source :
-
Biomacromolecules . Jul2007, Vol. 8 Issue 7, p2063-2071. 9p. - Publication Year :
- 2007
-
Abstract
- Supramolecular organized multilayers were constructed by multiwalled carbon nanotubes modified with ferrocene-derivatized poly(allylamine) redox polymer and glucose oxidase by electrostatic self-assembly. From the analysis of voltammetric signals and fluorescence results, a linear increment of the coverage of enzyme per bilayer was estimated, which demonstrated that the multilayer is constructed in a spatially ordered manner. The cyclic voltammograms obtained from the indium tin oxide (ITO) electrodes coated by the (Fc-PAHCNT/GOx)nmultilayers revealed that bioelectrocatalytic response is directly correlated to the number of deposited bilayers; that is, the sensitivity is tunable by controlling the number of bilayers associated with ITO electrodes. The incorporation of redox-polymer-functionalized carbon nanotubes (CNT) into enzyme films resulted in a 6−10-fold increase in the glucose electrocatalytic current; the bimolecular rate constant of FADH2oxidation (wiring efficiency) was increased up to 12-fold. Impedance spectroscopy data have yielded the electron diffusion coefficient (De) of this nanostructure to be over 10-8cm2s-1, which is typically higher than those systems without CNT by at least a factor of 10, indicating that electron transport in the new supramolecular architecture was enhanced by communication of the redox active site of enzyme, redox polymer, and CNT. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15257797
- Volume :
- 8
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 25860210
- Full Text :
- https://doi.org/10.1021/bm061049l