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Molecular characterization of the herpes simplex virus 1 (HSV-1) homologues, UL25 to UL30, in duck enteritis virus (DEV)
- Source :
-
Gene . Oct2007, Vol. 401 Issue 1/2, p88-96. 9p. - Publication Year :
- 2007
-
Abstract
- Abstract: A 16.6-kilo-base pair (kb) sequence was amplified from the duck enteritis virus (DEV) clone-03 strain genome using ‘targeted gene walking polymerase chain reaction (PCR)’. Seven complete open reading frames (ORFs) were predicted, and designated herpes simplex virus 1 (HSV-1) homologues, unique long (UL) 25, UL26, UL26.5, UL27, UL28, UL29, and UL30. Sequence analysis revealed that the arrangement of seven genes in DEV clone-03 strain was collinear to that from HSV-1. In addition, mRNA transcription orientation was identical to the HSV-1 genes. While UL25, UL26, and UL26.5 shared the same poly A signal, the UL27 and UL28 genes overlapped by 211bp nucleotides and shared the same 3′ transcription terminus. UL26.5, an in-frame ORF of UL26, was co-terminal with UL26 at its 3′-end. We predicted that the gene arrangement in the unique long segment of the DEV clone-03 was identical to that in HSV-1, particularly in the region from UL25 to UL30 gene. Phylogenetic trees of the putative proteins encoded by these seven genes showed that UL27, UL28, and UL30 had a close evolutionary relationship with the Mardivirus, however, the other four proteins exhibited close relationships with the Simplexvirus or Varicellovirus, indicating that the DEV clone-03 should be placed into a single cluster within the subfamily Alphaherpesvirinae. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 401
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 26488344
- Full Text :
- https://doi.org/10.1016/j.gene.2007.06.022