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Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 8/21/2007, Vol. 104 Issue 34, p13738-13743. 6p. 6 Graphs. - Publication Year :
- 2007
-
Abstract
- Aconitase (Aco1p) is a multifunctional protein: It is an enzyme of the tricarboxylic acid cycle. In animal cells, Aco1p also is a cytosolic protein binding to mRNAs to regulate iron metabolism. In yeast, Aco1p was identified as a component of mtDNA nucleoids. Here we show that yeast Acoip protects mtDNA from excessive accumulation of point mutations and SSDNA breaks and suppresses reductive recombination of mtDNA. Aconitase binds to both ds- and ssDNA, with a preference for GC-containing sequences. Therefore, mitochondria are opportunistic organelles that seize proteins, such as metabolic enzymes, for construction of the nucleoid, an mtDNA maintenance/segregation apparatus. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA-ligand interactions
*DNA
*NUCLEOIDS
*CELL nuclei
*NUCLEIC acids
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 104
- Issue :
- 34
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 26541878
- Full Text :
- https://doi.org/10.1073/pnas.0703078104