Back to Search
Start Over
PIP2-dependent inhibition of M-type (Kv7.2/7.3) potassium channels: direct on-line assessment of PIP2 depletion by Gq-coupled receptors in single living neurons.
- Source :
-
Pflügers Archiv: European Journal of Physiology . Oct2007, Vol. 455 Issue 1, p115-124. 10p. 1 Color Photograph, 1 Diagram, 4 Graphs. - Publication Year :
- 2007
-
Abstract
- The open state of M(Kv7.2/7.3) potassium channels is maintained by membrane phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2). They can be closed on stimulating receptors that induce PI(4,5)P2 hydrolysis. In sympathetic neurons, closure induced by stimulating M1-muscarinic acetylcholine receptors (mAChRs) has been attributed to depletion of PI(4,5)P2, whereas closure by bradykinin B2-receptors (B2-BKRs) appears to result from formation of IP3 and release of Ca2+, implying that BKR stimulation does not deplete PI(4,5)P2. We have used a fluorescently tagged PI(4,5)P2-binding construct, the C-domain of the protein tubby, mutated to increase sensitivity to PI(4,5)P2 changes (tubby-R332H-cYFP), to provide an on-line read-out of PI(4,5)P2 changes in single living sympathetic neurons after receptor stimulation. We find that the mAChR agonist, oxotremorine-M (oxo-M), produces a near-complete translocation of tubby-R332H-cYFP into the cytoplasm, whereas bradykinin (BK) produced about one third as much translocation. However, translocation by BK was increased to equal that produced by oxo-M when synthesis of PI(4,5)P2 was inhibited by wortmannin. Further, wortmannin ‘rescued’ M-current inhibition by BK after Ca2+-dependent inhibition was reduced by thapsigargin. These results provide the first direct support for the view that BK accelerates PI(4,5)P2 synthesis in these neurons, and show that the mechanism of BKR-induced inhibition can be switched from Ca2+ dependent to PI(4,5)P2 dependent when PI(4,5)P2 synthesis is inhibited. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NEURONS
*CELLS
*NERVOUS system
*POTASSIUM channels
*ION channels
Subjects
Details
- Language :
- English
- ISSN :
- 00316768
- Volume :
- 455
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Pflügers Archiv: European Journal of Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 26543009
- Full Text :
- https://doi.org/10.1007/s00424-007-0259-6