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Androgenesis as a means of dissecting complex genetic and physiological controls: selecting useful gene combinations for breeding freezing tolerant grasses.
- Source :
-
Euphytica . Nov2007, Vol. 158 Issue 3, p337-345. 9p. 1 Diagram, 2 Graphs. - Publication Year :
- 2007
-
Abstract
- The advantages of androgenesis from Lolium × Festuca hybrids as a means towards enhanced gene expression and capture of rare genetic variation are reviewed. New evidence is presented for the technique’s use in combination with introgression-mapping for targeting Festuca-derived genes for enhanced freezing-tolerance. As a starting point, a dihaploid genotype derived by androgenesis from a Lolium multiflorum × Festuca pratensis amphiploid (2 n = 4 x = 28) hybrid cultivar is used as female parent in a backcross breeding programme with L. multiflorum (2 n = 2 x = 14). A derivative of the backcross␣breeding programme was a genotype of L.␣multiflorum (2 n = 2 x = 14) incorporating a F.␣pratensis introgression on chromosome 4 that was more freezing-tolerant than Lolium. New evidence of the importance in Lolium and Festuca species of the adaptive capabilities of Photosystem II (PSII) in relation to subsequent freezing-tolerance, is presented. Non-photochemical quenching (NPQ) mechanisms for expulsion of excess light energy during cold acclimation are found in F. pratensis but not in L. multiflorum. Screens of a backcross population derived from an initial dihaploid genotype ( n + n = 14) produced by androgenesis from a L. multiflorum × F.␣pratensis amphiploid, indicate a direct relationship between cold acclimation induced increases in NPQ and freezing-tolerance. Preliminary evidence of a role for genes found on chromosome 4 of F. pratensis for increased NPQ expression, is presented. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142336
- Volume :
- 158
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Euphytica
- Publication Type :
- Academic Journal
- Accession number :
- 27258382
- Full Text :
- https://doi.org/10.1007/s10681-006-9240-2