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Use of a genetic cholera toxin B subunit/allergen fusion molecule as mucosal delivery system with immunosuppressive activity against Th2 immune responses
- Source :
-
Vaccine . Dec2007, Vol. 25 Issue 50, p8395-8404. 10p. - Publication Year :
- 2007
-
Abstract
- Abstract: Induction of peripheral tolerance can be facilitated when the antigen is linked to the B subunit of cholera toxin (CTB), an efficient mucosal carrier. In the present study, a genetic fusion molecule of Bet v 1 and CTB was produced to test whether mucosal application of this construct would lead to suppression of Th2 responses. Intranasal pretreatment of BALB/c mice with rCTB-Bet v 1 prior to allergic sensitisation with the allergen significantly decreased IgE but markedly increased allergen-specific IgG2a levels in sera as well as IFN-γ production of splenocytes. This Th1 shift was supported by an increased T-bet/GATA3 mRNA ratio. IL-5 production within the airways was suppressed after the pretreatment with rCTB-Bet v 1, while local allergen-specific IgA antibodies were markedly enhanced by pretreatment with the construct. Upregulation of Foxp3, IL-10 and TGF-β mRNA expression was detected in splenocytes after pretreatment with unconjugated allergen but not with the fusion molecule, indicating that antigen conjugation to a mucosal carrier modifies the immunomodulating properties of an antigen/allergen. [Copyright &y& Elsevier]
- Subjects :
- *CHOLERA
*VIBRIO infections
*GRAM-negative bacterial diseases
*IMMUNE response
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Volume :
- 25
- Issue :
- 50
- Database :
- Academic Search Index
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 27703307
- Full Text :
- https://doi.org/10.1016/j.vaccine.2007.10.003