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A low perfusion rate microreactor for continuous monitoring of enzyme characteristics: application to glucose oxidase.
- Source :
-
Analytical & Bioanalytical Chemistry . Nov2007, Vol. 389 Issue 6, p2029-2033. 5p. 1 Diagram, 1 Chart, 2 Graphs. - Publication Year :
- 2007
-
Abstract
- This report describes a versatile and robust microreactor for bioactive proteins physically immobilized on a polyether sulfone filter. The potential of the reactor is illustrated with glucose oxidase immobilized on a filter with a cut-off value of 30 kDa. A flow-injection system was used to deliver the reactants and the device was linked on-line to an electrochemical detector. The microreactor was used for on-line preparation of apoglucose oxidase in strong acid and its subsequent reactivation with flavin adenine dinucleotide. In addition we describe a miniaturized version of the microreactor used to assess several characteristics of femtomole to attomole amounts of glucose oxidase. A low negative potential over the electrodes was used when ferrocene was the mediator in combination with horseradish peroxidase, ensuring the absence of oxidation of electro-active compounds in biological fluids. A low backpressure at very low flow rates is an advantage, which increases the sensitivity. A variety of further applications of the microreactor are suggested. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROREACTORS
*OXIDASES
*FLAVINS
*GLUCOSE
*ELECTROCHEMICAL sensors
*ENZYMES
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 389
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27734003
- Full Text :
- https://doi.org/10.1007/s00216-007-1596-1