PROTEÍNAS RELACIONADAS CON LA DEFENSA EN LA INTERACCIÓN Sporisorium scitamineum - CAÑA DE AZÚCAR.

Laboratory experiments were conducted at the National Centre for Animal and Plant Health during 2001-2005. In this paper we determined super oxide dismutase iso-enzyme patterns and transcripts sequenced from PRs on the Sporisorium scitamineum - sugarcane interaction, which were inoculated buds of both varieties Ja60-5 (susceptible) and M31/45 (resistant) with a suspension of pathogen teliospores. Native electrophoresis separation was detected in two acidic isozymes, one was induced in high level resistant varieties in comparison with the other at basal constitutive level for both varieties. The cADN-AFLP analysis permitted the obtaining of sequences of PRs homologues to a glucanasas (PR2), quitinasas (PR3) and osmotina (PR5) differentially induced on resistant variety. These results confirm that the superoxido dismutase enzyme is involved in signal pathway on this interaction; and PRs induction on induced defence response and their possible use as biochemical and molecular marker on the Sugarcane Breeding Program. [ABSTRACT FROM AUTHOR]